bioSensAll™ is a highly dynamic and flexible platform that can be easily adapted to address all your questions and needs.
The assay has been successfully used with ligands of diverse biochemical nature (i.e., small molecules, peptides, biologics). All applications of this technology are based on the following basic protocol and are compatible with 96- and 384-well plate formats.
- Cells are co-transfected with vectors coding for the untagged GPCR of interest and the desired biosensor and then seeded into microplates (96- or 384-well) .
- 48 hours following the transfection, compounds are incubated with the cells and biosensor activity (i.e., BRET) is measured.
To date, a total of over 60 GPCRs have been either fully or partially profiled using bioSensAll™ technology. For numerous receptors, the signaling signatures of multiple ligands have been defined.
A few validated applications of bioSensAll™
Constitutive receptor activity and inverse agonist pharmacology measured with bioSensAll™
Characterization of receptor agonists and antagonists using bioSensAll™
Characterization of ligand signaling signatures and biased agonism with bioSensAll™
Measurement of pathway-specific signaling kinetics in real-time with bioSensAll™
Ligand profiling in cell lines of different tissular origin with bioSensAll™
Characterization and comparison of receptor orthologues using bioSensAll™