The bioSensAll platform includes BRET-based biosensors for monitoring both G protein-dependent and β-arrestin-mediated signaling downstream of G protein-coupled receptors (GPCRs).

These biosensors are designed to measure:

  • Activation of 14 distinct heterotrimeric G proteins as well as β-arrestins 1 and 2
  • Second messenger generation
    • cAMP
    • diacylglycerol (DAG)
    • phosphatidylinositol (3,4,5)-trisphosphate (PIP3)
  • Engagement of downstream effector proteins
    • RhoA
    • PKC activation
  • Receptor localization and trafficking (plasma membrane vs. early endosomes)
  • … more under development

Biosensors at the heart of the bioSensAll platform are constructed using different strategies . These biosensors can be broadly divided into the following functional categories:

A) Heterotrimeric G protein activation biosensors

I. G alpha/gamma-based G protein activation biosensors (GABY)

These multimolecular sensors were designed to monitor the conformational changes that occur within the heterotrimeric G protein complex upon Gα activation and effector interaction. They can be used to measure specific activation of each Gα subtype.

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II. G alpha plasma membrane (GAPL) biosensors

These multimolecular sensors detect plasma membrane recruitment of an effector protein that interacts with Gα subunits upon G protein activation. They can be used to measure specific activation of each Gα subtype.

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B) ß-arrestin biosensors

I. Beta-arrestin membrane recruitment biosensors

These multimolecular sensors detect the recruitment of ß-arrestin 1 or ß-arrestin 2 to the plasma membrane following receptor engagement.

A similar strategy is available to detect ß-arrestin recruitment to early endosomes.

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II. Beta-arrestin double brilliance conformation biosensors

These unimolecular biosensors are used to monitor conformational changes occurring within β-arrestins 1 or 2 upon GPCR activation.

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C) Second messenger biosensors

I. Diacylglycerol (DAG) biosensors

This multimolecular biosensor detects the generation of DAG at the plasma membrane following receptor activation.

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II. cAMP biosensor

This unimolecular biosensor serves to detect intracellular cAMP levels following GPCR stimulation.

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III. Phosphatidylinositol (3,4,5)-trisphosphate (PIP3) biosensor

This multimolecular biosensor detects the generation of PIP3 at the plasma membrane following receptor activation.

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D) Effector biosensors

I. RhoA activation biosensor

This multimolecular sensor detects ligand-induced activation of the small G protein RhoA at the plasma membrane.

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II. Protein kinase C (PKC) activation biosensor

This unimolecular biosensor serves to detect the activity of protein kinase C (PKC).

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E) Receptor trafficking biosensors

The multimolecular receptor trafficking and localization biosensors allow for real-time monitoring of plasma membrane and endosome localization of GPCRs or ß-arrestins following their activation. Further, these sensors can be used in a complementary fashion to study plasma membrane-endosome trafficking kinetics.

I. Plasma membrane localization biosensor

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The multimolecular receptor trafficking and localization biosensors allow for real-time monitoring of plasma membrane and endosome localization of GPCRs following their activation. Further, these sensors can be used in a complementary fashion to study plasma membrane-endosome trafficking kinetics.

II. Early endosome localization biosensor

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For access to any of the biosensors, please contact us at info@biosensall.com